Advantages of bispecific hybridomas in one-step immunocytochemistry and immunoassays.

Production of murine hybrid-hybridomas secreting bispecific monoclonal antibodies for use in urease-based immunoassays.

To produce bispecific antibodies (BiAbs) for enzyme immunoassay (EIA) to replace antibody-enzyme conjugates, we developed a panel of 8-azaguanine/ouabain-resistant anti-urease variant hybridoma cell lines for use in hybridoma-hybridoma fusions. These variants represent mouse immunoglobulin subclasses IgM, IgG1, IgG2a, and IgG2b and have growth rates equal to those of the parental hybridomas. We...

Multiplexed Bead Immunoassays: Advantages and Limitations in Pediatrics

When performing an immunoassay, a specific antibody is used to identify and quantify the concentration of target molecules or analytes in complex samples, such as serum or urine. The use of monoclonal antibodies, highly specific, was a great improvement in these assays. Currently, the combination of these specific antibodies with different fluorophores and novel detection technologies has allow...

One step forward

One-step immunoassays for free (unbound) hormones: the effects of tracer binding by serum proteins.

In binding assays for determination of free (non-protein-bound) hormones in serum or plasma, the influence of the measuring system on the original analyte concentration in the sample must be considered. In one- or single-step free-hormone immunoassays, the labeled analyte or analog-tracer not only is bound to the antibody, it also is bound, to some extent, to serum proteins. The dependence of t...

Dimensional accuracy of three impression materials by one-step and two-step impression techniques: An in vitro study

Introduction: An accurate impression is essential to create a well-fitted dental prosthesis. This study aimed to compare the dimensional accuracy of three elastomeric materials using one-step and two-step impression techniques. Methods: In this study, 20 impressions were fabricated for each Vinyl siloxane ether (Identium), condensation silicone (Speedex), and a...